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MB Sample ID: SA221021

Local Sample ID:brain_44058
Subject ID:SU002384
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

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Treatment:

Treatment ID:TR002396
Treatment Summary:According to the National Institutes of Health Guide for the Care and Use of Laboratory Animals, all animals involved in this study were cared for, and protocols were reviewed and approved by the Anhui Laboratory Animal Care Committee. The specific pathogen-free (SPF) seven-week-old male Sprague Dawley (SD) rats (weight 233 ± 5 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd and used in this study. The environmental conditions were set at 21-26°C with a relative humidity of 50 ± 10% and a 12/12 h light/dark cycle. Food and tap water were provided ad libitum, and body weights were recorded daily. After one week of acclimatization, rats were randomly assigned to the groups of non-stressed control (n = 6) or the stressed (n = 7). For restraint stress, rats were individually placed in a ventilated plastic tube restrainer for 120 minutes, using a previously modified method. According to the general protocol, control rats were left undistributed in a home cage and allowed to contact each other without food and water.
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