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MB Sample ID: SA305798

Local Sample ID:28
Subject ID:SU002935
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Sample Preparation:

Sampleprep ID:SP002941
Sampleprep Summary:Fasting serum samples taken during the cold or RT exposures were stored at -80 °C and thawed in an ice water bath. Four hundred microliters of ice-cold acetonitrile (ACN) was pipetted to 96-well filter plates (Captiva ND Plate, 0.2 μm PP, Agilent Technologies, Santa Clara, CA, United States) after which 100 μL of plasma or quality control (QC) was added. The solution was mixed by pipetting up and down, filtrated by centrifugation (700 RCF, 4 °C, 5 min) and collected to a 96-1 well plate (96 deep well plate natural,Thermo Scientific). QC was serum sample prepared by pooling an aliquot from all analysed serum samples.