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MB Sample ID: SA300995

Local Sample ID:20220202_B6_3j_M2_48_1_636
Subject ID:SU002914
Subject Type:Other organism
Subject Species:Biofilm microbial community

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Sample Preparation:

Sampleprep ID:SP002920
Sampleprep Summary:Briefly, freeze-dried biofilms (1 mg) were diluted in 10 µL of cold 75% methanol acidified with 0.1% formic acid and sonicated on ice during 30 s, at 80 % of the maximum intensity (SONICS Vibra Cell, Newton, CT, USA; 130 Watts, 20 kHz). The homogenates were then centrifuged at 4°C (12,000 g: 10 min). The supernatants were then collected and stored in the dark at -20°C.
Processing Storage Conditions:-20℃