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MB Sample ID: SA300833

Local Sample ID:HPLT_S3
Subject ID:SU002911
Subject Type:Bacteria
Subject Species:Microbacterium sediminis YLB-01

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Sample Preparation:

Sampleprep ID:SP002917
Sampleprep Summary:After culturing the YLB-01 cells, each 100 mL of the culture was transferred into a 250-mL centrifuge bottle and centrifuged at 4°C (6000 g, 5 min). The supernatant was carefully decanted, and the cell pellets were rapidly cooled to -40°C using 100 mL of a buffer composed of a 3:2 methanol/water mixture containing 0.85% (wt/vol) NaCl. The mixture was centrifuged again at 4°C (6000 g, 5 min). The cell pellets were washed three times with 5 mL of cold phosphate-buffered saline (PBS) and centrifuged at 4°C (6000 g, 5 min) after each wash. Finally, the cell pellets were stored at -80°C until further use. Initially, 600 μL of a cold extraction buffer consisting of a 1:1 mixture of distilled water and acetonitrile was added to homogenize the samples. The mixtures were then sonicated on wet ice for 180 cycles, with each cycle consisting of 2 seconds of ultrasound followed by a 3-second pause. After centrifugation at 4°C (12000 g, 10 min), the supernatants were collected and lyophilized, resulting in an extract powder that was stored at -80°C for further analysis.
Processing Storage Conditions:Described in summary
Extract Storage:Described in summary