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MB Sample ID: SA300802
Local Sample ID: | G31-Con-31A3A |
Subject ID: | SU002909 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 6 months |
Weight Or Weight Range: | 27-43 |
Gender: | Male |
Animal Animal Supplier: | The Jackson Laboratory |
Species Group: | Mammals |
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Sample Preparation:
Sampleprep ID: | SP002915 |
Sampleprep Summary: | Serum samples from each mouse were thawed and 20 μL was consumed to extract metabolites for each mice. Metabolite extraction was facilitated by adding 180 μL methanol containing stable isotope-labeled chemicals ([D5]-glutamine, [D2]-𝛾-aminobutyric acid, [D3]-tryptophan, [D2]-indole-3-propionic acid, [D2]-indole-3-acetic acid, [D13]-acetylcholine in 500 nM; [D4]-serotonin in 250 nM; and [D4]-kynurenic acid in 50 nM), and incubated under -20°C for 1 hr, followed by centrifugation under 15,000 ×g, 4°C for 10 minutes to collect the supernatant (150 μL). The supernatant was dried by SpeedVac® and reconstituted with 100 μL 2% acetonitrile in water. |