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MB Sample ID: SA258281
| Local Sample ID: | 5694 |
| Subject ID: | SU002670 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
| Species Group: | Mammals |
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Sample Preparation:
| Sampleprep ID: | SP002676 |
| Sampleprep Summary: | Total lipids were extracted from 40mg of pulverized hearts under liquid nitrogen using in methyl tert-butyl ether (MTBE) and ethyl acetate after spiking with the following internal standards lysophosphatidylcholine (LPC) 13:0, phosphatidylcholine (PC) 19:0/19:0, PC14:0/14:0, phosphatidylserine (PS) 12:0/12:0, phosphatidylglycerol (PG) 15:0/15:0 and phosphatidylethanolamine (PE) 17:0/17:0). Supernatants were evaporated using a Speed Vacuum concentrator overnight, dissolved in methanol/chloroform (2:1) and aliquots (50 µL) were stored at -80ºC until use. |
| Sampleprep Protocol Filename: | Sample preparation protocol of untargeted lipidomic analysis |
| Processing Storage Conditions: | -80℃ |
