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MB Sample ID: SA235222
Local Sample ID: | H8 |
Subject ID: | SU002431 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl/6 |
Age Or Age Range: | 20 weeks (at euthanasia) |
Gender: | Male and female |
Animal Animal Supplier: | Jackson Labratories |
Animal Light Cycle: | Standard light dark cycle |
Animal Feed: | PicoLab Rodent Diet 20, 20% protein |
Species Group: | Mammals |
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Sample Preparation:
Sampleprep ID: | SP002437 |
Sampleprep Summary: | Once euthanized, tissues were dissected and harvested for various techniques. For metabolomics, humeri samples underwent homogenization/crushing, extracted with methanol:acetone, and were then subjected to rounds of vortexing and centrifugation. Once metabolites were isolated, samples were dried via vacuum concentration and resuspended with acetonitrile:water for LC-MS analysis. |