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MB Sample ID: SA234001
Local Sample ID: | FB0179 |
Subject ID: | SU002426 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
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Sample Preparation:
Sampleprep ID: | SP002432 |
Sampleprep Summary: | The order of all test samples is randomly selected before the preparation. 10 mg lyophilized feces were homogenized with 25μL water and extracted with 185μL cold ACN-Methanol (8/2, v/v). After centrifugation, 30 μL supernatant was used to derivatization with 20 μL freshly prepared derivative reagents on a Biomek 4000 workstation (Biomek 4000, Beckman Coulter, Inc., Brea, California, USA), followed by mixing with internal standards. Subsequently, the derivatized samples and serial dilutions of derivatized stock standards were randomly analyzed and quantitated by an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) system (ACQUITY UPLC-Xevo TQ-S, Waters Corp., Milford, MA, USA). |