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MB Sample ID: SA227123
Local Sample ID: | M17TP2 |
Subject ID: | SU002399 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
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Sample Preparation:
Sampleprep ID: | SP002405 |
Sampleprep Summary: | Samples were dispensed into a 96-well plate, dried at room temperature for 30 min and then 50 µL of phenylisothiocyanate (PITC) was added. After a further 20 min incubation at room temperature samples were dried under nitrogen for 1 h. Ammonium acetate (3 mM) was added, and the plate was shaken at room temperature for 30 min and centrifuged for 2 min at 500 g. Each well then had 150 µL transferred from it to a 96 deep well plate along with and equal volume of dH2O. |