Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA227123

Local Sample ID:	M17TP2
Subject ID:	SU002399
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammals

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP002405
Sampleprep Summary:	Samples were dispensed into a 96-well plate, dried at room temperature for 30 min and then 50 µL of phenylisothiocyanate (PITC) was added. After a further 20 min incubation at room temperature samples were dried under nitrogen for 1 h. Ammonium acetate (3 mM) was added, and the plate was shaken at room temperature for 30 min and centrifuged for 2 min at 500 g. Each well then had 150 µL transferred from it to a 96 deep well plate along with and equal volume of dH2O.

Sampleprep ID:

SP002405

Sampleprep Summary:

Samples were dispensed into a 96-well plate, dried at room temperature for 30 min and then 50 µL of phenylisothiocyanate (PITC) was added. After a further 20 min incubation at room temperature samples were dried under nitrogen for 1 h. Ammonium acetate (3 mM) was added, and the plate was shaken at room temperature for 30 min and centrifuged for 2 min at 500 g. Each well then had 150 µL transferred from it to a 96 deep well plate along with and equal volume of dH2O.