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MB Sample ID: SA216441
| Local Sample ID: | FIT01-074-pos |
| Subject ID: | SU002337 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 6-17 years |
| Weight Or Weight Range: | Non-exacerbation-prone IQR: 30-68 kg; Exacerbation-prone IQR: 42-63 kg |
| Height Or Height Range: | Non-exacerbation-prone IQR: 131-165 cm; Exacerbation-prone IQR: 140-163 cm |
| Gender: | Male and female |
| Human Race: | 18 white, 189 Black, 7 more than one race, 1 Asian |
| Human Ethnicity: | 2 Hispanic, 113 Not Hispanic |
| Human Medications: | 92 on high-dose inhaled corticosteroids; 94 on long-acting beta adrenergic receptor agonists; 111 on Montelukast |
| Human Smoking Status: | 24 exposed to tobacco smoke |
| Human Inclusion Criteria: | 1) current or historical evidence of >=12% reversibility in forced expiratory volume in one second (FEV1) relative to baseline after bronchodilator administration; 2) airway hyperresponsiveness to methacholine, with a provocative concentration of methacholine causing a 20% drop in FEV1 |
| Human Exclusion Criteria: | 1) premature birth before 35 weeks of gestation; 2) other chronic airway disorders that could mimic asthma. |
| Species Group: | Mammals |
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Sample Preparation:
| Sampleprep ID: | SP002343 |
| Sampleprep Summary: | 50 uL of plasma was mixed with 200 uL of a 50:50 mixture of methanol and acetonitirile, vortexed, incubated on ice for 30 min, then centrifuged at 20,000 g for 10 min at 4 *C. 200 uL of the supernatant was collected for analysis. |
| Extraction Method: | 50:50 methanol:acetonitrile for a 1:2:2 samples:methanol:acetonitrile final |
| Extract Cleanup: | centrifuged at 20,000 g for 10 min at 4 *C |
| Extract Storage: | -80℃ |
