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MB Sample ID: SA211839
Local Sample ID: | AD5 media |
Subject ID: | SU002299 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 50-90 yrs |
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Sample Preparation:
Sampleprep ID: | SP002305 |
Sampleprep Summary: | Metabolites from frozen cell pellets were extracted at 1e6 cells/mL in cold 5:3:2 MeOH:acetonitrile:water. Metabolites from supernatant samples (10 uL) were extracted with 240 uL of the above extraction solution. Samples were vortexed 30 min at 4 degrees C then supernatants clarified by centrifugation (10 min, 10,000 g, 4 degrees C). |