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MB Sample ID: SA209961

Local Sample ID:CM02-029
Subject ID:SU002270
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Sample Preparation:

Sampleprep ID:SP002276
Sampleprep Summary:Metabolite extraction solution (50% methanol, 30% acetonitrile, 20% water, 5uM valine-d8 as internal standard) was added to (10-20mg) frozen small intestine tissue samples at an extraction ratio of 25ul/mg on dry ice. Samples were then homogenized using a Precellys 24 tissue homogenizer (Bertin technologies). The resulting sample suspension was vortexed, mixed at 4oC in a Thermomixer for 15 min at 1,500 rpm and then centrifuged at 16,000 x g for 20 min at 4oC. The supernatant was collected for LC-MS analysis.