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MB Sample ID: SA204347
Local Sample ID: | HSC_Cultured_12h_1 |
Subject ID: | SU002210 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP002216 |
Sampleprep Summary: | To get the corresponding derivatives, the dried aliquots were incubated with 20 µL 2% (w/v) methoxyamine hydrochloride (226904, Sigma-Aldrich) in pyridine for 60 min at 37 °C, and silylated by 30 µL of N-Methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS, 18162-48-6, Regis Technologies) for 30 min at 45 °C. The corresponding derivatives were analyzed by GC-MS using the Trace 1310 gas chromatograph (Thermo Fisher) with the DB-35ms column (Agilent Technologies) connected to the Q ExactiveTM GC OrbitrapTM GC-MS/MS system (Thermo Fisher). |