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MB Sample ID: SA192282
Local Sample ID: | NHF-21 |
Subject ID: | SU002126 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
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Sample Preparation:
Sampleprep ID: | SP002132 |
Sampleprep Summary: | Serum samples (100µl) should be properly thawed and spin at 5K rpm for one minute, added with 100 % MeOH (300µl, in1:3 ratio) and vortex well (30s), incubated on ice for 10 minutes and then centrifuged at 10K rpm for 10 minutes at 4℃. Equally, transfer the supernatant into two different well-labeled tubes and then speed vacuum dry and stored at -80℃. One set of the tubes is used for the analysis of polar metabolites and the other one is used for the non-polar metabolites. For the reconstitution of polar metabolites, 50% ACN (50 µl) is added into the vacuum dried sample and for nonpolar 15% of MeOH (50 µl) is used. After the addition of reconstitutes, vortex well for 10 min and centrifuged at 10K rpm for 10 minutes at 4℃ and transfer the supernatant (approx. 40-45 µl) into MS vials. Remove 10µl form every sample containing MS vials and pooled together into a 15 ml tube to use as QC. Then, the samples were stored at -80℃ for further analysis. |