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MB Sample ID: SA181876

Local Sample ID:Et_stem_3
Subject ID:SU002012
Subject Type:Plant
Subject Species:Equisetum arvense;Equisetum hyemale;Equisetum telmateia

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Sample Preparation:

Sampleprep ID:SP002018
Sampleprep Summary:Five biological replicates (separate plants) were harvested at the same time of day for below-ground rhizomes and above-ground stems of vegetative shoots. Samples were snap-frozen in liquid nitrogen and freeze-dried (aerial parts for 5 days, rhizomes for 7 days). Lyophilized material was submerged in liquid nitrogen, homogenized using mortar and pestle.
Extraction Method:Frozen tissue homogenate (30 mg per sample) was transferred to a 2 ml reaction tube and extracted with 1 ml of 80 % aqueous methanol (containing 10 mg/l anthracene-9-carboxylic acid as internal standard) by vigorous shaking (VX-2500 multi-tube vortexer, VWR Scientific, South Plainfield, NY, USA) for 10 min and subsequent sonication for 20 min (FS30 ultrasonic cleaner, Fisher Scientific, Hampton, NY, USA). Following centrifugation for 10 min at 13,000 × g (5415 microfuge, Eppendorf, Enfield, CT, USA), the supernatant was filtered through 0.22 μm polypropylene syringe filter tips, and the flow-through collected in plastic inserts for 2 ml reaction vials.