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MB Sample ID: SA175941

Local Sample ID:7
Subject ID:SU001970
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male

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Sample Preparation:

Sampleprep ID:SP001976
Sampleprep Summary:Biofluids were prepared as previously described (Pannkuk et al. 2018;2020). Urine (20 μl) was deproteinated with 50% acetonitrile (80 μl) containing internal standards (2 μM debrisoquine sulfate, 30 μM 4-nitrobenzoic acid), incubated on ice for 10 min, vortexed for 30 seconds, and centrifuged for 10 min (10,000 x g, 4°C). Serum (5 μl) was prepared as above but was deproteinated with 66% acetonitrile (195 μl). A quality control (QC) sample was prepared by mixing 1 μl from each sample and prepared as above.
Processing Storage Conditions:-80℃
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