Return to study ST001667 main page
MB Sample ID: SA152293
Local Sample ID: | 5--4 |
Subject ID: | SU001744 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001750 |
Sampleprep Summary: | Microcentrifuge tubes with 30 mg of frozen liver tissue had 500 uL of ice-cold acetone and three Zirconia beads added to them. Tissues were lysed and homogenized, then sonicated and centrifuged. The supernatant was transferred to a new tube and 500 uL of ice-cold MeOH/H2O was added to the pellet. The pellet was sonicated and centrifuged again. The supernatant was combined with the acetone fraction and vacuum dried. Dried pellets were resuspended in acetonitrile/H2O with 1% Formic Acid, vortexed, sonicated, and centrifuged. 180 uL of eluate was used for LCMS analysis. |