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MB Sample ID: SA300810
Local Sample ID: | G31-Con-31A4B |
Subject ID: | SU002909 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 6 months |
Weight Or Weight Range: | 27-43 |
Gender: | Male |
Animal Animal Supplier: | The Jackson Laboratory |
Species Group: | Mammals |
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Combined analysis:
Analysis ID | AN004558 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | m/z |
MS:
MS ID: | MS004305 |
Analysis ID: | AN004558 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The mass spectrometry was set to scan under the positive mode with the sheath gas, auxiliary gas, and sweep gas set to flow rates of 50, 13, and 3 psi, respectively. With the spray voltage set to 3.5 kV, the capillary and auxiliary gas heating temperature were respectively controlled to 263°C and 425°C to fully scan across m/z 70 to 1,000. The resolution was set to 70,000 FWHM (m/z 200). The automatic gain control (AGC) and the maximal injection time (MIT) was set to 2×105 and 50 msec, respectively. Routine mass calibrations were conducted before and after the sample analysis. The samples were blocked-randomized for the injection order. Quality control samples for the serum analytes were prepared by pooling the aliquots of each sample. Method blank samples were prepared for the serum samples by surrogating the biospecimen with water and following the same experimental procedures. If MS/MS spectrums were to be collected, the parallel reaction monitoring (PRM) mode was used, with the isolation width, AGC, and MIT set to 1.2, 3×105 and 100 msec, respectively, at the resolution of 17,500 FWHM (m/z 200). |
Ion Mode: | POSITIVE |