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MB Sample ID: SA244682

Local Sample ID:Norm. Area: 088_0003_02G_R_A10xKOxFx02conxHILICneg_29SEP21_CEL_MOU_LUM_0000_04AB_15MIN_KP_KP_01.raw (F146)
Subject ID:SU002535
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male and female

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Combined analysis:

Analysis ID AN003984 AN003985 AN003986 AN003987
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Thermo Hypersil GOLD C18 (150 x 2.1mm, 1.9um) Thermo Hypersil GOLD C18 (150 x 2.1mm, 1.9um) Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Fusion Tribrid Orbitrap Thermo Fusion Tribrid Orbitrap Thermo Fusion Tribrid Orbitrap Thermo Fusion Tribrid Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak Area Peak Area Peak Area Peak area

MS:

MS ID:MS003718
Analysis ID:AN003984
Instrument Name:Thermo Fusion Tribrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:MS1 data were acquired over a mass range of 70 to 1500 m/z in the orbitrap operated at a resolution of 60,000. MS/MS data were acquired in AcquireX mode using the iterative precursor exclusion workflow, with a stepped HCD collision energy at 20, 35 and 50, at a resolution of 30,000 in the orbitrap
Ion Mode:POSITIVE
  
MS ID:MS003719
Analysis ID:AN003985
Instrument Name:Thermo Fusion Tribrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:MS1 data were acquired over a mass range of 70 to 1500 m/z in the orbitrap operated at a resolution of 60,000. MS/MS data were acquired in AcquireX mode using the iterative precursor exclusion workflow, with a stepped HCD collision energy at 20, 35 and 50, at a resolution of 30,000 in the orbitrap
Ion Mode:NEGATIVE
  
MS ID:MS003720
Analysis ID:AN003986
Instrument Name:Thermo Fusion Tribrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:MS1 data were acquired over a mass range of 70 to 1500 m/z in the orbitrap operated at a resolution of 60,000. MS/MS data were acquired in AcquireX mode using the iterative precursor exclusion workflow, with a stepped HCD collision energy at 20, 35 and 50, at a resolution of 30,000 in the orbitrap
Ion Mode:POSITIVE
  
MS ID:MS003721
Analysis ID:AN003987
Instrument Name:Thermo Fusion Tribrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:MS1 data were acquired over a mass range of 70 to 1500 m/z in the orbitrap operated at a resolution of 60,000. MS/MS data were acquired in AcquireX mode using the iterative precursor exclusion workflow, with a stepped HCD collision energy at 20, 35 and 50, at a resolution of 30,000 in the orbitrap
Ion Mode:NEGATIVE
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