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MB Sample ID: SA226658
| Local Sample ID: | FIT2-LKO_03 |
| Subject ID: | SU002389 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003763 | AN003764 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Thermo Accucore C30 (250 x 2.1mm,3um) | Thermo Accucore C30 (250 x 2.1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Area | Area |
MS:
| MS ID: | MS003506 |
| Analysis ID: | AN003763 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry data analysis was performed using LipidSearch version 4.1 SP (Thermo Fisher Scientific). The results were exported to R-Studio where quality control was performed using pairwise correlations between replicates, a principal component analysis comparing sample groups, as well as retention time plot analysis to verify elution clustering within lipid classes. All identified lipids were included for subsequent analyses if they fulfilled the following LipidSearch-based criteria: 1) reject equal to zero, 2) main grade A OR main grade B AND APvalue<0.01 for at least three replicates, and 3) no missing values across all samples. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003507 |
| Analysis ID: | AN003764 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry data analysis was performed using LipidSearch version 4.1 SP (Thermo Fisher Scientific). The results were exported to R-Studio where quality control was performed using pairwise correlations between replicates, a principal component analysis comparing sample groups, as well as retention time plot analysis to verify elution clustering within lipid classes. All identified lipids were included for subsequent analyses if they fulfilled the following LipidSearch-based criteria: 1) reject equal to zero, 2) main grade A OR main grade B AND APvalue<0.01 for at least three replicates, and 3) no missing values across all samples. |
| Ion Mode: | NEGATIVE |
