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MB Sample ID: SA210074
Local Sample ID: | codY 3h_3 |
Subject ID: | SU002273 |
Subject Type: | Bacteria |
Subject Species: | Staphylococcus aureus |
Taxonomy ID: | 1280 |
Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003581 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Waters Acquity I-Class |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 6500+ QTrap |
Ion Mode | UNSPECIFIED |
Units | CPS |
MS:
MS ID: | MS003338 |
Analysis ID: | AN003581 |
Instrument Name: | ABI Sciex 6500+ QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | QTRAP6500+ operated in polarity switching mode was used for targeted quantitation of amino acids through Multiple Reaction Monitoring (MRM) process. Electrospray ionization (ESI) parameters were optimized are as follows: electrospray ion voltage of -4200V and 5500V in negative and positive mode respectively, source temperature of 400°C, curtain gas of 35, and gas 1 and 2 of 40 and 40 psi, respectively. Compound specific parameters were optimized for each compound using manual tuning. These parameters are declustering potential (DP) were 65V and -60V in positive and negative mode respectively, entrance potential (EP) was set at 10V and -10V in positive and negative mode respectively and collision cell exit potential (CXP) was maintained at 10V and -10V in positive and negative mode respectively. MRM data was analysed using Multiquant software. |
Ion Mode: | UNSPECIFIED |