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MB Sample ID: SA210074

Local Sample ID:codY 3h_3
Subject ID:SU002273
Subject Type:Bacteria
Subject Species:Staphylococcus aureus
Taxonomy ID:1280
Gender:Not applicable

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Combined analysis:

Analysis ID AN003581
Analysis type MS
Chromatography type HILIC
Chromatography system Waters Acquity I-Class
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 6500+ QTrap
Ion Mode UNSPECIFIED
Units CPS

MS:

MS ID:MS003338
Analysis ID:AN003581
Instrument Name:ABI Sciex 6500+ QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:QTRAP6500+ operated in polarity switching mode was used for targeted quantitation of amino acids through Multiple Reaction Monitoring (MRM) process. Electrospray ionization (ESI) parameters were optimized are as follows: electrospray ion voltage of -4200V and 5500V in negative and positive mode respectively, source temperature of 400°C, curtain gas of 35, and gas 1 and 2 of 40 and 40 psi, respectively. Compound specific parameters were optimized for each compound using manual tuning. These parameters are declustering potential (DP) were 65V and -60V in positive and negative mode respectively, entrance potential (EP) was set at 10V and -10V in positive and negative mode respectively and collision cell exit potential (CXP) was maintained at 10V and -10V in positive and negative mode respectively. MRM data was analysed using Multiquant software.
Ion Mode:UNSPECIFIED
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