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MB Sample ID: SA209901
| Local Sample ID: | CM02b-062 |
| Subject ID: | SU002270 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
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Collection:
| Collection ID: | CO002263 |
| Collection Summary: | Small intestines were dissected and washed with PBS. Small pieces of approximately ~0.5 cm were isolated from proximal (post stomach) small intestine and snap frozen on dry ice and stored at -80°C until further processing. The remaining small intestinal tissue was washed in PBS to remove faeces and cut longitudinally. Intestinal epithelial cells were isolated by sequential incubation of intestinal tissue in pre-heated 1 mM dithiothreitol and 1.5 mM EDTA solutions at 37oC while shaking. Intestinal epithelial cell pellet was frozen at -80°C until further processing. Intestinal epithelial cell (IEC) pellet was frozen at -80oC for further processing. |
| Sample Type: | Intestine |
