Return to study ST002125 main page

MB Sample ID: SA204340

Local Sample ID:HSC_Cultured_1
Subject ID:SU002210
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Select appropriate tab below to view additional metadata details:


Collection ID:CO002203
Collection Summary:5×10^5 indicated cells were incubated with 100 µM stable [13C,15N] amino acids (MSK-A2-US-1.2, Cambridge Isotope Laboratories) for indicated time and centrifuged at 500 g for 5 min at 4 °C. The pelleted cells were extracted in 500 µl ice-cold Acetonitrile: Isopropyl Alcohol: water (3:3:2 v/v/v) and aliquoted as three technical replicates. The extracts were vortexed for 5 min at 4 °C and centrifuged at 14,000 g for 2 min at 4 °C. The supernatants were dried by vacuum spin for subsequent derivatization and stored at -20 ℃.
Sample Type:Bone marrow