Return to study ST003069 main page
MB Sample ID: SA332115
| Local Sample ID: | MXP500L-0-5711_1036867631_19_0_1_1_00_1036866439_16_R30V3 |
| Subject ID: | SU003184 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005026 | AN005027 |
|---|---|---|
| Chromatography ID | CH003798 | CH003798 |
| MS ID | MS004765 | MS004766 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | ExionLC AD chromatography system (AB Sciex, Framingham, MA, USA) | ExionLC AD chromatography system (AB Sciex, Framingham, MA, USA) |
| Column | MxP Quant 500 Column System (Biocrates Part No: 21117) | MxP Quant 500 Column System (Biocrates Part No: 21117) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 6500+ QTrap | ABI Sciex 6500+ QTrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | µM | µM |
Chromatography:
| Chromatography ID: | CH003798 |
| Chromatography Summary: | Measurements were carried out using LC-MS/MS and flow injection (FIA)-MS/MS analyses on a Sciex 6500+ series mass spectrometer coupled to an ExionLC AD chromatography system (AB Sciex, Framingham, MA, USA), utilizing the Analyst 1.7.1 software with hotfix 1 (also AB SCIEX). All required standards, quality controls and eluents were included in the kit, as well as the chromatographic column for the LC-MS/MS analysis part. LC1_pos: The UHPLC autosampler and column oven are held at 10 °C and 50 °C, respectively. The injection volume was 5 µL. Eluent A is water (0.2% formic acid) and eluent B is acetonitrile (0.2% formic acid). Gradient run time of 5.8 mins. Remain at 0% B for 0.25 min, linear gradient to 12% B at 1.5 min and 17.5% B at 2.7 min. Further linear increase to 50% B at 4 mins and 100% B at 4.5 min, where it remains until 5 min. Reduction to 0% at 5.1 min and remaining at 0% until 5.8 min. Flow rate of 0.8 mL/min until 4.5 min, then increase to 1 mL/min until 4.7 min and remaining until 5.1 min. Finally, reduction to 0.8 mL/min until 5.8 min. |
| Instrument Name: | ExionLC AD chromatography system (AB Sciex, Framingham, MA, USA) |
| Column Name: | MxP Quant 500 Column System (Biocrates Part No: 21117) |
| Column Temperature: | 50°C |
| Flow Gradient: | Gradient run time of 5.8 mins. Remain at 0% B for 0.25 min, linear gradient to 12% B at 1.5 min and 17.5% B at 2.7 min. Further linear increase to 50% B at 4 mins and 100% B at 4.5 min, where it remains until 5 min. Reduction to 0% at 5.1 min and remaining at 0% until 5.8 min. Flow rate of 0.8 mL/min until 4.5 min, then increase to 1 mL/min until 4.7 min and remaining until 5.1 min. Finally, reduction to 0.8 mL/min until 5.8 min. |
| Flow Rate: | 0.8 mL/min |
| Solvent A: | 100% Water; 0.2% FA |
| Solvent B: | 100% ACN; 0.2% FA |
| Chromatography Type: | Reversed phase |
