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MB Sample ID: SA308603

Local Sample ID:Neg_WT_4
Subject ID:SU002962
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

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Combined analysis:

Analysis ID AN004668 AN004669
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Vanquish Flex UHPLC Systems Thermo Vanquish Flex UHPLC Systems
Column HILICON iHILIC-(P) Classic (100 x 2.1mm,5um) HILICON iHILIC-(P) Classic (100 x 2.1mm,5um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Orbitrap ID-X Tribrid Thermo Orbitrap ID-X Tribrid
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH003514
Instrument Name:Thermo Vanquish Flex UHPLC Systems
Column Name:HILICON iHILIC-(P) Classic (100 x 2.1mm,5um)
Column Temperature:45
Flow Gradient:0–1 min: 90% B, 1–12 min: 90-35% B, 12–12.5 min: 35-25% B, 12.5–14.5 min: 25% B
Flow Rate:250 uL/min
Solvent A:20 mM ammonium bicarbonate, 0.1% ammonium hydroxideand 2.5 μM medronic acid in water:acetonitrile (95:5)
Solvent B:acetonitrile:water (95:5)
Chromatography Type:HILIC
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