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MB Sample ID: SA308492
Local Sample ID: | ID_111 |
Subject ID: | SU002957 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Species Group: | Mammals |
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Combined analysis:
Analysis ID | AN004663 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1220 Infinity |
Column | Agilent Zorbax Eclipse Plus C18 (150 x 4.6mm,3.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | intesity |
Chromatography:
Chromatography ID: | CH003509 |
Chromatography Summary: | The binary mobile phases were water 0.5% formic acid with 5 mM of ammonium formate (A), and acetonitrile (B). Their gradient elution started with 20% (B) for 5 min, then linearly increased to 100% (B) in 30 min and kept constant for 8 min in 100% (B). The eluent was restored to the initial conditions in 4 minutes to re-equilibrate the column and held for the remaining 8 minutes. The flow rate was kept at 0.5 mL min-1. The injection volume for analysis was 3 μL, and the column temperature was set at 35 °C. |
Instrument Name: | Agilent 1220 Infinity |
Column Name: | Agilent Zorbax Eclipse Plus C18 (150 x 4.6mm,3.5um) |
Column Temperature: | 35 |
Flow Gradient: | gradient elution started with 20% (B) for 5 min, then linearly increased to 100% (B) in 30 min and kept constant for 8 min in 100% (B). The eluent was restored to the initial conditions in 4 minutes to re-equilibrate the column and held for the remaining 8 minutes. |
Flow Rate: | 0.5 mL/min |
Solvent A: | 100% water; 0.5% formic acid; 5 mM of ammonium formate |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |