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MB Sample ID: SA307477

Local Sample ID:WTa_UT_Lys1
Subject ID:SU002950
Subject Type:Bacteria
Subject Species:Bacillus subtilis
Taxonomy ID:1423
Genotype Strain:168
Age Or Age Range:NA
Weight Or Weight Range:NA
Height Or Height Range:NA
Gender:Not applicable
Cell Biosource Or Supplier:NA
Cell Strain Details:NA
Subject Comments:NA
Cell Primary Immortalized:NA
Cell Passage Number:NA
Cell Counts:NA
Species Group:NA

Select appropriate tab below to view additional metadata details:


Combined analysis:

Analysis ID AN004647 AN004648
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Infinity II Agilent 1290 Infinity II
Column Microsolv Diamond hydride (150 x 2.1mm,4um) Microsolv Diamond hydride (150 x 2.1mm,4um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode NEGATIVE POSITIVE
Units Area under the curve Area under the curve

Chromatography:

Chromatography ID:CH003497
Chromatography Summary:Liquid chromatography (Agilent 1290 Infinity II) was used for the formic acid method.
Methods ID:NA
Methods Filename:NA
Instrument Name:Agilent 1290 Infinity II
Column Name:Microsolv Diamond hydride (150 x 2.1mm,4um)
Column Pressure:NA
Column Temperature:25
Flow Gradient:0–2 min, 85% B; 3–5 min, 80% B; 6–7 min, 75%; 8–9 min, 70% B; 10–11 min, 50% B; 11–14 min 20% B; 14–24 min 5% B followed by a 10 min re-equilibration period at 85% B
Flow Rate:0.4
Injection Temperature:25
Solvent A:Milli Q H2O + 0.2% Formic Acid
Solvent B:Acetonitrile + 0.2% Formic Acid
Chromatography Type:Normal phase
Solvent C:NA
  
Chromatography ID:CH003498
Chromatography Summary:Liquid chromatography (Agilent 1290 Infinity II) was used for the formic acid method.
Instrument Name:Agilent 1290 Infinity II
Column Name:Microsolv Diamond hydride (150 x 2.1mm,4um)
Column Temperature:25
Flow Gradient:0–2 min, 85% B; 3–5 min, 80% B; 6–7 min, 75%; 8–9 min, 70% B; 10–11 min, 50% B; 11–14 min 20% B; 14–24 min 5% B followed by a 10 min re-equilibration period at 85% B
Flow Rate:0.4
Solvent A:Milli Q H2O + 0.2% Formic Acid
Solvent B:Acetonitrile + 0.2% Formic Acid
Chromatography Type:Normal phase
Solvent C:NA
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