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MB Sample ID: SA258272

Local Sample ID:5980
Subject ID:SU002670
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male
Species Group:Mammals

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Combined analysis:

Analysis ID AN004233
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode POSITIVE
Units Counts

Chromatography:

Chromatography ID:CH003141
Methods Filename:LC-MS and data processing protocol of untargeted lipidomic analysis
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:0–2 min, 50% B; 2–11 min, 50–85% B; 11–33 min, 85–94% B; 33–35 min, 94–98% B, 35–38 min; 98–99.5% B; 38–82 min, 98–99.5% B; 82–83 min, 99.5–50% B, plus an equilibration of 8 min
Flow Rate:0.45 ml/min
Solvent A:100% water; 0.2% formic acid; 10 mM ammonium formate
Solvent B:55% ethanol,/35% acetonitrile/10% MTBE, 55:35:10; 0.2% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase
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