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MB Sample ID: SA235224
Local Sample ID: | H12 |
Subject ID: | SU002431 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl/6 |
Age Or Age Range: | 20 weeks (at euthanasia) |
Gender: | Male and female |
Animal Animal Supplier: | Jackson Labratories |
Animal Light Cycle: | Standard light dark cycle |
Animal Feed: | PicoLab Rodent Diet 20, 20% protein |
Species Group: | Mammals |
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Combined analysis:
Analysis ID | AN003826 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1290 |
Column | Cogent Diamond Hydride (150 × 2.1mm,4um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6538 QTOF |
Ion Mode | POSITIVE |
Units | Peak Intensity |
Chromatography:
Chromatography ID: | CH002831 |
Chromatography Summary: | Samples were analyzed using an Agilent 1290 UPLC coupled to an Agilent 6538 QTOF. All samples were run in normal phase using a Cogent Diamond Hydride HILIC column (150 x 2.1 mm; 100Å pore size on a 4µm particle size). Solvents: 0.1% formic acid in water (A), 0.1% formic acid in acetonitrile (B). Elution gradient: 100 to 25% solvent B over 17 minutes, 0 to 70% solvent A over 20 minutes, at 21 minutes, B increases from 25% to 100% till 25 minutes while A decreases from 70% to 0% till 25 minutes. The last 4 minutes act as a wash. Total run time = 25 minute method (per sample) |
Instrument Name: | Agilent 1290 |
Column Name: | Cogent Diamond Hydride (150 × 2.1mm,4um) |
Flow Gradient: | 100 to 25% solvent B over 17 minutes, 0 to 70% solvent A over 20 minutes, at 21 minutes, B increases from 25% to 100% till 25 minutes while A decreases from 70% to 0% till 25 minutes. The last 4 minutes act as a wash. |
Flow Rate: | 0.400 mL/min |
Injection Temperature: | 4C |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Analytical Time: | 25 minutes |
Chromatography Type: | HILIC |