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MB Sample ID: SA232064

Local Sample ID:14
Subject ID:SU002421
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female
Cell Strain Details:The SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH (ATCC HTB-11), which was established in 1970 from a metastatic bone tumor from a 4-year-old cancer patient.

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Combined analysis:

Analysis ID AN003810 AN003811
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Shimadzu Nexera X2 Shimadzu Nexera X2
Column Waters XBridge Amide (100 x 4.6mm,3.5um) Waters XBridge Amide (100 x 4.6mm,3.5um)
MS Type ESI ESI
MS instrument type hybrid triple quadrupole/linear ion trap hybrid triple quadrupole/linear ion trap
MS instrument name ABI Sciex 6500 QTrap ABI Sciex 6500 QTrap
Ion Mode POSITIVE NEGATIVE
Units nmol lipid per mg protein nmol lipid per mg protein

Chromatography:

Chromatography ID:CH002819
Chromatography Summary:Chromatographic separation was performed on a XBridge amide column (150 mm × 4.6 mm, 3.5 μm; Waters) maintained at 35°C using mobile phase A [1 mM ammonium acetate in water-acetonitrile 5:95 (v/v)] and mobile phase B [1 mM ammonium acetate in water-acetonitrile 50:50 (v/v)] in the following gradient: (0-6 min: 0% B - 6% B; 6-10 min: 6% B - 25% B; 10-11 min: 25% B - 98% B; 11-13 min: 98% B - 100% B; 13-19 min: 100% B; 19-24 min: 0% B) at a flow rate of 0.7 mL/min, which was increased to 1.5 mL/min from 13 minutes onwards.
Instrument Name:Shimadzu Nexera X2
Column Name:Waters XBridge Amide (100 x 4.6mm,3.5um)
Column Temperature:35
Flow Gradient:0-6 min: 0% B - 6% B; 6-10 min: 6% B - 25% B; 10-11 min: 25% B - 98% B; 11-13 min: 98% B - 100% B; 13-19 min: 100% B; 19-24 min: 0% B
Flow Rate:0.7 mL/min, which was increased to 1.5 mL/min from 13 minutes onwards
Solvent A:5% water/95% acetonitrile; 1 mM ammonium acetate
Solvent B:50% water/50% acetonitrile; 1 mM ammonium acetate
Chromatography Type:HILIC
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