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MB Sample ID: SA216396
| Local Sample ID: | FIT01-197-pos |
| Subject ID: | SU002337 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 6-17 years |
| Weight Or Weight Range: | Non-exacerbation-prone IQR: 30-68 kg; Exacerbation-prone IQR: 42-63 kg |
| Height Or Height Range: | Non-exacerbation-prone IQR: 131-165 cm; Exacerbation-prone IQR: 140-163 cm |
| Gender: | Male and female |
| Human Race: | 18 white, 189 Black, 7 more than one race, 1 Asian |
| Human Ethnicity: | 2 Hispanic, 113 Not Hispanic |
| Human Medications: | 92 on high-dose inhaled corticosteroids; 94 on long-acting beta adrenergic receptor agonists; 111 on Montelukast |
| Human Smoking Status: | 24 exposed to tobacco smoke |
| Human Inclusion Criteria: | 1) current or historical evidence of >=12% reversibility in forced expiratory volume in one second (FEV1) relative to baseline after bronchodilator administration; 2) airway hyperresponsiveness to methacholine, with a provocative concentration of methacholine causing a 20% drop in FEV1 |
| Human Exclusion Criteria: | 1) premature birth before 35 weeks of gestation; 2) other chronic airway disorders that could mimic asthma. |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003677 | AN003678 |
|---|---|---|
| Chromatography ID | CH002727 | CH002727 |
| MS ID | MS003428 | MS003429 |
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Vanquish Horizon Binary ultrahigh performance liquid chromatography system | Vanquish Horizon Binary ultrahigh performance liquid chromatography system |
| Column | SeQuant ZIC-HILIC | SeQuant ZIC-HILIC |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002727 |
| Chromatography Summary: | Equilibration time was 4 min at 90% B, with 3.5 min at 0.35 mL/min, then 0.5 min at 0.25 mL/min. After sample injection, a gradient ran from 90% to 20% B over 17.5 min at 0.25 mL/min, then flow was increased to 0.35 mL/min for 1 min and held at 20% B at 0.35 mL/min for 3.5 min. |
| Instrument Name: | Vanquish Horizon Binary ultrahigh performance liquid chromatography system |
| Column Name: | SeQuant ZIC-HILIC |
| Column Pressure: | 15-180 bar |
| Column Temperature: | 40 |
| Flow Rate: | 0.25-0.35 mL/min |
| Sample Injection: | 2 uL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 1.85-14.5 min |
| Chromatography Type: | HILIC |
