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MB Sample ID: SA212856

Local Sample ID:batch14_MT_20210808_051
Subject ID:SU002319
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN003643
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Scientific Vanquish Horizon UHPLC system
Column HILIC column, AccucoreTM-150-Amide (50 x 2.1mm,2.6um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Orbitrap ID-X tribrid
Ion Mode POSITIVE
Units m/z values

Chromatography:

Chromatography ID:CH002697
Chromatography Summary:Mobile phase: 10 mM ammonium acetate in 95% acetonitrile containing 0.1% acetic acid as mobile phase A and 10 mM ammonium acetate in 50% acetonitrile containing 0.1% acetic acid as mobile phase B were used for HILIC method. Mobile phase gradient: For HILIC acquisition, following gradient was applied at a flow rate of 0.55 ml/min: 0-0.1 min: 0% B, 0.10-5.0 min: 98% B, 5.00-5.50 min: 0% B and 4.5 min for cleaning and equilibration of column.For RP column, following gradient was applied at a flow rate of 0.4 ml/min: 0-0.1 min: 0% B, 0.10-1.9 min: 60% B, 1.9-5.0 min: 98% B, 5.00-5.10 min: 0% B and 4.9 min cleaning and column equilibration.
Instrument Name:Thermo Scientific Vanquish Horizon UHPLC system
Column Name:HILIC column, AccucoreTM-150-Amide (50 x 2.1mm,2.6um)
Column Temperature:45
Flow Gradient:0-0.1 min: 0% B, 0.10-5.0 min: 98% B, 5.00-5.50 min: 0% B and 4.5 min for cleaning and equilibration of column.
Flow Rate:0.55 ml/min
Solvent A:95% acetonitrile/5% water; 0.1% acetic acid; 10 mM ammonium acetate
Solvent B:50% acetonitrile/50% water; 0.1% acetic acid; 10 mM ammonium acetate
Chromatography Type:HILIC
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