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MB Sample ID: SA212725
Local Sample ID: | batch15_MT_20210809_187 |
Subject ID: | SU002319 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
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Combined analysis:
Analysis ID | AN003643 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Scientific Vanquish Horizon UHPLC system |
Column | HILIC column, AccucoreTM-150-Amide (50 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Orbitrap ID-X Tribrid |
Ion Mode | POSITIVE |
Units | m/z values |
Chromatography:
Chromatography ID: | CH002697 |
Chromatography Summary: | Mobile phase: 10 mM ammonium acetate in 95% acetonitrile containing 0.1% acetic acid as mobile phase A and 10 mM ammonium acetate in 50% acetonitrile containing 0.1% acetic acid as mobile phase B were used for HILIC method. Mobile phase gradient: For HILIC acquisition, following gradient was applied at a flow rate of 0.55 ml/min: 0-0.1 min: 0% B, 0.10-5.0 min: 98% B, 5.00-5.50 min: 0% B and 4.5 min for cleaning and equilibration of column.For RP column, following gradient was applied at a flow rate of 0.4 ml/min: 0-0.1 min: 0% B, 0.10-1.9 min: 60% B, 1.9-5.0 min: 98% B, 5.00-5.10 min: 0% B and 4.9 min cleaning and column equilibration. |
Instrument Name: | Thermo Scientific Vanquish Horizon UHPLC system |
Column Name: | HILIC column, AccucoreTM-150-Amide (50 x 2.1mm,2.6um) |
Column Temperature: | 45 |
Flow Gradient: | 0-0.1 min: 0% B, 0.10-5.0 min: 98% B, 5.00-5.50 min: 0% B and 4.5 min for cleaning and equilibration of column. |
Flow Rate: | 0.55 ml/min |
Solvent A: | 95% acetonitrile/5% water; 0.1% acetic acid; 10 mM ammonium acetate |
Solvent B: | 50% acetonitrile/50% water; 0.1% acetic acid; 10 mM ammonium acetate |
Chromatography Type: | HILIC |