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MB Sample ID: SA210089

Local Sample ID:codY ccpA 3h_1
Subject ID:SU002273
Subject Type:Bacteria
Subject Species:Staphylococcus aureus
Taxonomy ID:1280
Gender:Not applicable

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Combined analysis:

Analysis ID AN003581
Analysis type MS
Chromatography type HILIC
Chromatography system Waters Acquity I-Class
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS instrument type QTRAP
MS instrument name ABI Sciex 6500+
Units CPS


Chromatography ID:CH002648
Chromatography Summary:Triple-quadrupole-ion trap hybrid mass spectrometer viz., QTRAP6500+ (Sciex, USA) connected with ultra-performance liquid chromatography I-class (UPLC) system procured from Waters, USA was used for the metabolite analysis. The chromatographic separation was performed by liquid chromatography using XBridge Amide (150 × 2.1mm ID; 3.5µm particle size, Waters, USA) analytical column and a binary solvent system with a flow rate of 0.4 ml/min. A guard XBridge Amide column (20 × 2.1mm ID; 3.5µm particle size, Waters, USA) was connected in front of analytical column. Mobile phase A was composed of 10 mM ammonium acetate, 10mM ammonium hydroxide containing 5% acetonitrile in LC-MS grade water; mobile phase B was 100% LC-MS grade acetonitrile.
Instrument Name:Waters Acquity I-Class
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Flow Rate:0.4 ml/min
Solvent A:5% acetonitrile/95% water; 10 mM ammonium acetate; 10 mM ammonium hydroxide
Solvent B:100% acetonitrile
Chromatography Type:HILIC