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MB Sample ID: SA192160

Local Sample ID:D-79
Subject ID:SU002126
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN003325 AN003326 AN003327 AN003328
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters Xbridge BEH HILIC (75 x 2.1mm,2.5um) Waters Xbridge BEH HILIC (75 x 2.1mm,2.5um) Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Fusion Orbitrap Thermo Fusion Orbitrap Thermo Fusion Orbitrap Thermo Fusion Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units peak height peak height peak height peak height

Chromatography:

Chromatography ID:CH002464
Chromatography Summary:For hydrophilic interaction (HILIC) chromatography, 5 µl of the prepared samples were injected for analysis. An ultimate 3000 UPLC XBridge BHE Amide column (2.1 x 100 mm, 2.5 µm; Waters Corporation) was used and maintained at 35℃ for separation. The mobile phase was delivered at 0.35mL/min. The mobile phase A consisted of 20mM ammonium acetate in the water of pH 9.0 and mobile phase B was 100% acetonitrile. The elution gradient is used as follows: 0 min-85% B, 2 min-10% B, 14 min- 10% B, 14.1 min- 85% B, 16 min- 85%B.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Xbridge BEH HILIC (75 x 2.1mm,2.5um)
Column Temperature:35
Flow Gradient:0 min-85% B, 2 min-10% B, 14 min- 10% B, 14.1 min- 85% B, 16 min- 85%B.
Flow Rate:0.35mL/min
Solvent A:100% water; 20 mM ammonium acetate, pH 9.0
Solvent B:100% acetonitrile
Chromatography Type:HILIC
  
Chromatography ID:CH002465
Chromatography Summary:For reversed-phase (RP) chromatographic analyses, 5 µL of prepared samples were injected. The separation was performed using a UPLC ultimate 3000 using the HSS T3 column (2.1x 100mm, 1.7 µm; Waters Corporation) at 40℃. The mobile phase was delivered at 300µL/min and consisted of eluent A (water with 0.1%formic acid) and eluent B (acetonitrile with 0.1% formic acid), delivered in a gradient profile: 0min-1% B, 1min-15%B, 4min-35% B, 7min-95% B, 9 min- 95% B, 10min-1% B, 14 min- 1% B.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Flow Gradient:0min-1% B, 1min-15%B, 4min-35% B, 7min-95% B, 9 min- 95% B, 10min-1% B, 14 min- 1% B
Flow Rate:300µL/min
Solvent A:100% water; 0.1%formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
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