Return to study ST000618 main page
MB Sample ID: SA034354
| Local Sample ID: | HR04_2 |
| Subject ID: | SU000641 |
| Subject Type: | Human Follicular Fluid |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | female |
| Human Exclusion Criteria: | cancer, endometriosis, premature ovarian failure |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000948 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Ultima Quadrupole-Time of Flight - Waters Corporation |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Ultima QTOF |
| Ion Mode | POSITIVE |
| Units | mass intensity |
Chromatography:
| Chromatography ID: | CH000675 |
| Chromatography Summary: | Liquid Chromatography was performed by using a chromatographer Acquity with a reverse phase column (Acquity UPCL HSS 1,8 µm, 2.1x100 mm - Waters Corporation, MA, USA), at 35 °C. The solvents for the elution were: A- H2O with formic acid 0.1% and B- ACN with formic acid 0.1%. The initial gradient was composed of 80% of A and 20% of B, during 1 minute. Next, the gradient was linearly changed for 50% of A and 50% of B, during the next minute. In the sequence, the gradient was changed for 35% of A and 65% of B during 2 minutes. The gradient was then inverted, considering 20% of A and 80% of B, during 3 minutes. For the next 10 minutes, the gradient was 3% of A and 97% of B. The initial condition was reestablished for the column washing during 1 minute. The total run time was 17 minutes and the solvents flux was 0.5 mL/min and the total amount of sample used per run was 5 µL. |
| Instrument Name: | Ultima Quadrupole-Time of Flight - Waters Corporation |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 35 °C |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
