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MB Sample ID: SA313036
| Local Sample ID: | tgg1_3 |
| Subject ID: | SU002979 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Species Group: | Plants |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002979 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Species Group: | Plants |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| tgg1_3 | SA313036 | FL037400 | tgg1 | Genotype |
Collection:
| Collection ID: | CO002972 |
| Collection Summary: | Root VOCs and soil VOCs were trapped as described here (Schulz-Bohm, et al, 2018, "Calling from distance: attraction of soil bacteria by plant root volatiles", The ISME Journal, 12(5), pp.1252-1262.). Briefly, 1 Tenax cartridge was fitted into the side arms of the glass pots in such a way that their opening was exposed toward the plant roots/soil. The other arm received a constant airflow from an aerator. VOCs were sampled for 40 hours and immediately analyzed by thermal desorption-gas chromatography‒mass spectrometry. |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR002988 |
| Treatment Summary: | No specific treatment. |
Sample Preparation:
| Sampleprep ID: | SP002985 |
| Sampleprep Summary: | After 40 hours of VOC trapping, Tenax cartridges were immediately subjected to thermal desorption. |
Combined analysis:
| Analysis ID | AN004700 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent DB-FFAP (60m x 250um, 0.25um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5977B |
| Ion Mode | POSITIVE |
| Units | % relative peak abundance |
Chromatography:
| Chromatography ID: | CH003540 |
| Chromatography Summary: | Chromatography GC column used was DB-FFAP, 60m length, 250um inner diameter, 0.25um film thickness) |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent DB-FFAP (60m x 250um, 0.25um) |
| Column Temperature: | NA |
| Flow Gradient: | NA |
| Flow Rate: | NA |
| Solvent A: | NA |
| Solvent B: | NA |
| Chromatography Type: | GC |
MS:
| MS ID: | MS004447 |
| Analysis ID: | AN004700 |
| Instrument Name: | Agilent 5977B |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | Analysis was performed using MassHunter Qualified software to extract and integrate peat spectra to profile the root volatiles from WT and mutant lines. The data from only soil was considered as the blank and was subtracted from the rVOCs data. Compounds were identified by using the library NIST 2020 (Agilent Technologies, Palo Alto, CA, USA) with minimum hit count of 65. The compounds that were found to be present in at least two biological replicates out of three were taken into consideration for further analysis. |
| Ion Mode: | POSITIVE |
