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MB Sample ID: SA313028

Local Sample ID:lox1_1
Subject ID:SU002979
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
Species Group:Plants

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Subject:

Subject ID:SU002979
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
Species Group:Plants

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
lox1_1SA313028FL037397lox1Genotype

Collection:

Collection ID:CO002972
Collection Summary:Root VOCs and soil VOCs were trapped as described here (Schulz-Bohm, et al, 2018, "Calling from distance: attraction of soil bacteria by plant root volatiles", The ISME Journal, 12(5), pp.1252-1262.). Briefly, 1 Tenax cartridge was fitted into the side arms of the glass pots in such a way that their opening was exposed toward the plant roots/soil. The other arm received a constant airflow from an aerator. VOCs were sampled for 40 hours and immediately analyzed by thermal desorption-gas chromatography‒mass spectrometry.
Sample Type:Plant

Treatment:

Treatment ID:TR002988
Treatment Summary:No specific treatment.

Sample Preparation:

Sampleprep ID:SP002985
Sampleprep Summary:After 40 hours of VOC trapping, Tenax cartridges were immediately subjected to thermal desorption.

Combined analysis:

Analysis ID AN004700
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Agilent DB-FFAP (60m x 250um, 0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5977B
Ion Mode POSITIVE
Units % relative peak abundance

Chromatography:

Chromatography ID:CH003540
Chromatography Summary:Chromatography GC column used was DB-FFAP, 60m length, 250um inner diameter, 0.25um film thickness)
Instrument Name:Agilent 7890B
Column Name:Agilent DB-FFAP (60m x 250um, 0.25um)
Column Temperature:NA
Flow Gradient:NA
Flow Rate:NA
Solvent A:NA
Solvent B:NA
Chromatography Type:GC

MS:

MS ID:MS004447
Analysis ID:AN004700
Instrument Name:Agilent 5977B
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:Analysis was performed using MassHunter Qualified software to extract and integrate peat spectra to profile the root volatiles from WT and mutant lines. The data from only soil was considered as the blank and was subtracted from the rVOCs data. Compounds were identified by using the library NIST 2020 (Agilent Technologies, Palo Alto, CA, USA) with minimum hit count of 65. The compounds that were found to be present in at least two biological replicates out of three were taken into consideration for further analysis.
Ion Mode:POSITIVE
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