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MB Sample ID: SA307477

Local Sample ID:WTa_UT_Lys1
Subject ID:SU002950
Subject Type:Bacteria
Subject Species:Bacillus subtilis
Taxonomy ID:1423
Genotype Strain:168
Age Or Age Range:NA
Weight Or Weight Range:NA
Height Or Height Range:NA
Gender:Not applicable
Cell Biosource Or Supplier:NA
Cell Strain Details:NA
Subject Comments:NA
Cell Primary Immortalized:NA
Cell Passage Number:NA
Cell Counts:NA
Species Group:NA

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU002950
Subject Type:Bacteria
Subject Species:Bacillus subtilis
Taxonomy ID:1423
Genotype Strain:168
Age Or Age Range:NA
Weight Or Weight Range:NA
Height Or Height Range:NA
Gender:Not applicable
Cell Biosource Or Supplier:NA
Cell Strain Details:NA
Subject Comments:NA
Cell Primary Immortalized:NA
Cell Passage Number:NA
Cell Counts:NA
Species Group:NA

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
WTa_UT_Lys1SA307477FL037042WTCU1065_untreatedGenotype

Collection:

Collection ID:CO002943
Collection Summary:All strains were grown in LB at 37 ˚C until 0.4 OD600 followed by 60 min treatment with or without 0.15 mM MnCl2 37 ˚C. Cells were pelleted and quenched by resuspending in chilled 0.6 ml of mixtures of acetonitrile:methanol:water (40:40:20).
Collection Protocol ID:NA
Collection Protocol Filename:NA
Collection Protocol Comments:NA
Sample Type:Bacterial cells
Collection Method:NA
Collection Location:NA
Collection Frequency:NA
Collection Duration:NA
Volumeoramount Collected:NA
Storage Conditions:-80℃
Collection Vials:Bead beating tubes
Storage Vials:NA
Additives:NA
Tissue Cell Identification:NA
Tissue Cell Quantity Taken:NA

Treatment:

Treatment ID:TR002959
Treatment Summary:All strains were treated without (control group) or with (treated group) 0.15 mM of MnCl2 for 60 min at 37 ˚C.
Treatment Protocol ID:NA
Treatment Protocol Filename:NA
Treatment Protocol Comments:NA
Treatment:NA
Treatment Compound:MnCl2 salt
Treatment Route:Direct in bacterial culture
Treatment Dose:0.15 mM
Treatment Dosevolume:NA
Treatment Doseduration:NA
Treatment Vehicle:Water
Cell Storage:-80
Cell Growth Container:Flask
Cell Growth Config:200 rpm
Cell Growth Rate:40-60 min
Cell Inoc Proc:NA
Cell Media:LB Media
Cell Envir Cond:NA
Cell Harvesting:acetonitrile:methanol:water (40:40:20)
Cell Pct Confluence:0.4 OD600
Cell Media Lastchanged:NA

Sample Preparation:

Sampleprep ID:SP002956
Sampleprep Summary:Samples were lysed using 0.1 mm Zirconia beads in a Precellys homogenizer. Lysed suspensions were centrifuged at 12,000 rpm for 8 min at 37 ˚C. Supernatants were passed through 0.22 um SpinX tube filter and flowthrough was collected for the LC-MS run.
Sampleprep Protocol ID:NA
Sampleprep Protocol Filename:NA
Sampleprep Protocol Comments:NA
Processing Method:NA
Processing Storage Conditions:On ice
Extraction Method:Beat beating
Extract Enrichment:NA
Extract Cleanup:NA
Extract Storage:4℃
Sample Resuspension:NA
Sample Derivatization:NA
Sample Spiking:NA
Subcellular Location:NA

Combined analysis:

Analysis ID AN004647 AN004648
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Infinity II Agilent 1290 Infinity II
Column Microsolv Diamond hydride (150 x 2.1mm,4um) Microsolv Diamond hydride (150 x 2.1mm,4um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode NEGATIVE POSITIVE
Units Area under the curve Area under the curve

Chromatography:

Chromatography ID:CH003497
Chromatography Summary:Liquid chromatography (Agilent 1290 Infinity II) was used for the formic acid method.
Methods ID:NA
Methods Filename:NA
Instrument Name:Agilent 1290 Infinity II
Column Name:Microsolv Diamond hydride (150 x 2.1mm,4um)
Column Pressure:NA
Column Temperature:25
Flow Gradient:0–2 min, 85% B; 3–5 min, 80% B; 6–7 min, 75%; 8–9 min, 70% B; 10–11 min, 50% B; 11–14 min 20% B; 14–24 min 5% B followed by a 10 min re-equilibration period at 85% B
Flow Rate:0.4
Injection Temperature:25
Solvent A:Milli Q H2O + 0.2% Formic Acid
Solvent B:Acetonitrile + 0.2% Formic Acid
Chromatography Type:Normal phase
Solvent C:NA
  
Chromatography ID:CH003498
Chromatography Summary:Liquid chromatography (Agilent 1290 Infinity II) was used for the formic acid method.
Instrument Name:Agilent 1290 Infinity II
Column Name:Microsolv Diamond hydride (150 x 2.1mm,4um)
Column Temperature:25
Flow Gradient:0–2 min, 85% B; 3–5 min, 80% B; 6–7 min, 75%; 8–9 min, 70% B; 10–11 min, 50% B; 11–14 min 20% B; 14–24 min 5% B followed by a 10 min re-equilibration period at 85% B
Flow Rate:0.4
Solvent A:Milli Q H2O + 0.2% Formic Acid
Solvent B:Acetonitrile + 0.2% Formic Acid
Chromatography Type:Normal phase
Solvent C:NA

MS:

MS ID:MS004394
Analysis ID:AN004647
Instrument Name:Agilent 6220 TOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Profinder B.08.00 Was used for the data analysis
Ion Mode:NEGATIVE
  
MS ID:MS004395
Analysis ID:AN004648
Instrument Name:Agilent 6220 TOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Profinder B.08.00 Was used for the data analysis
Ion Mode:POSITIVE
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