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MB Sample ID: SA305781

Local Sample ID:16
Subject ID:SU002935
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU002935
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
16SA305781FL036729coldTreatment

Collection:

Collection ID:CO002928
Collection Summary:Blood samples were either taken during the exposure to room temperature or during cold (subsequent to 2 hours of personalised cooling). More details are in the manuscript.
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002944
Treatment Summary:The exposure to cold for 2 hours under cooling blankets using a personalised cooling protocol

Sample Preparation:

Sampleprep ID:SP002941
Sampleprep Summary:Fasting serum samples taken during the cold or RT exposures were stored at -80 °C and thawed in an ice water bath. Four hundred microliters of ice-cold acetonitrile (ACN) was pipetted to 96-well filter plates (Captiva ND Plate, 0.2 μm PP, Agilent Technologies, Santa Clara, CA, United States) after which 100 μL of plasma or quality control (QC) was added. The solution was mixed by pipetting up and down, filtrated by centrifugation (700 RCF, 4 °C, 5 min) and collected to a 96-1 well plate (96 deep well plate natural,Thermo Scientific). QC was serum sample prepared by pooling an aliquot from all analysed serum samples.

Combined analysis:

Analysis ID AN004610 AN004611 AN004612 AN004613
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Agilent 1290 Agilent 1290 Agilent 1290 Agilent 1290
Column Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Agilent 6540 QTOF Agilent 6540 QTOF Agilent 6540 QTOF Agilent 6540 QTOF
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Area Area Area Area

Chromatography:

Chromatography ID:CH003468
Instrument Name:Agilent 1290
Column Name:Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm
Column Temperature:45
Flow Gradient:0–2.5 min: 100% B, 2.5–10 min: 100% B → 0% B; 10–10.01 min: 0% B → 100% B; 10.01–12.5 min: 100% B.
Flow Rate:0.6 mL/min.
Solvent A:50% acetonitrile with 20 mM ammonium formate and 0.25% formic acid, pH 3
Solvent B:90% acetonitrile with 20 mM ammonium formate, 0.25% formic acid, pH 3.
Chromatography Type:HILIC
  
Chromatography ID:CH003469
Instrument Name:Agilent 1290
Column Name:Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm
Column Temperature:50
Flow Gradient:2 to 100% B in 10 min; 100% B from 10 min to 14.5 min; 100% to 2% B, from 14.51 min to 16.5 min
Flow Rate:0.4 mL/min.
Solvent A:water with 0.1% (v/v) of formic acid
Solvent B:methanol with 0.1% (v/v) of formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004356
Analysis ID:AN004610
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent Technologies).
Ion Mode:POSITIVE
  
MS ID:MS004357
Analysis ID:AN004611
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent Technologies).
Ion Mode:NEGATIVE
  
MS ID:MS004358
Analysis ID:AN004612
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent Technologies).
Ion Mode:POSITIVE
  
MS ID:MS004359
Analysis ID:AN004613
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The data acquisition software was the MassHunter Acquisition B.05.01 (Agilent Technologies).
Ion Mode:NEGATIVE
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