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MB Sample ID: SA300995

Local Sample ID:20220202_B6_3j_M2_48_1_636
Subject ID:SU002914
Subject Type:Other organism
Subject Species:Biofilm microbial community

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Subject:

Subject ID:SU002914
Subject Type:Other organism
Subject Species:Biofilm microbial community

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
20220202_B6_3j_M2_48_1_636SA300995FL0365573Sampling day
20220202_B6_3j_M2_48_1_636SA300995FL036557fExperimental group
20220202_B6_3j_M2_48_1_636SA300995FL0365571 x 10-7Cobalt concentration

Collection:

Collection ID:CO002907
Collection Summary:Four weeks before the start of the experiment, the mature biofilms were collected by natural colonisation of glass slides (5 x 10 cm).
Sample Type:Microbial biofilm

Treatment:

Treatment ID:TR002923
Treatment Summary:A total of 15 microcosms were filled with 15 L of the Gave de Pau water; three for each of the five exposure conditions: 2 x 10-9 M (background concentration used as control), 1 x 10-7, 1 x 10-6, 5 x 10-6 and 1 x10-5 M Co. Cobalt (Cobalt Standard for ICP, 1000 mg·L-1, Supelco, Germany) was added in each microcosm and let equilibrating for 24 h (D-1). The microcosms were placed in one of the artificial streams of the facility in order to maintain the water temperature at 13.5 ± 1.0°C (Table S2). After one, three and seven days of exposure (D1, D3 and D7), slides were collected from each microcosm for metabolomics.

Sample Preparation:

Sampleprep ID:SP002920
Sampleprep Summary:Briefly, freeze-dried biofilms (1 mg) were diluted in 10 µL of cold 75% methanol acidified with 0.1% formic acid and sonicated on ice during 30 s, at 80 % of the maximum intensity (SONICS Vibra Cell, Newton, CT, USA; 130 Watts, 20 kHz). The homogenates were then centrifuged at 4°C (12,000 g: 10 min). The supernatants were then collected and stored in the dark at -20°C.
Processing Storage Conditions:-20℃

Combined analysis:

Analysis ID AN004564
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Thermo Acclaim Polar Advantage II (150 x 3mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Bruker maXis Impact qTOF
Ion Mode POSITIVE
Units Dalton

Chromatography:

Chromatography ID:CH003429
Instrument Name:Waters Acquity
Column Name:Thermo Acclaim Polar Advantage II (150 x 3mm,3um)
Column Temperature:40
Flow Gradient:5-90%
Flow Rate:300 microliter per minute
Solvent A:Water
Solvent B:ACN
Chromatography Type:Reversed phase

MS:

MS ID:MS004310
Analysis ID:AN004564
Instrument Name:Bruker maXis Impact qTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Metabolites were subsequently ionised and analysed using an electrospray ionization hybrid quadrupole time-of-flight high-resolution mass spectrometer (ESI-Qq-TOF Compact, Bruker) at 2 Hz speed on the 50–1500 m/z range on positive autoMS/MS mode between 2 and 8 Hz speed on the 50–1500 m/z range with information-dependent acquisition (IDA).
Ion Mode:POSITIVE
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