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MB Sample ID: SA300995
Local Sample ID: | 20220202_B6_3j_M2_48_1_636 |
Subject ID: | SU002914 |
Subject Type: | Other organism |
Subject Species: | Biofilm microbial community |
Species Group: | Microbes |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002914 |
Subject Type: | Other organism |
Subject Species: | Biofilm microbial community |
Species Group: | Microbes |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
20220202_B6_3j_M2_48_1_636 | SA300995 | FL036557 | 3 | Sampling day |
20220202_B6_3j_M2_48_1_636 | SA300995 | FL036557 | f | Experimental group |
20220202_B6_3j_M2_48_1_636 | SA300995 | FL036557 | 1 x 10-7 | Cobalt concentration |
Collection:
Collection ID: | CO002907 |
Collection Summary: | Four weeks before the start of the experiment, the mature biofilms were collected by natural colonisation of glass slides (5 x 10 cm). |
Sample Type: | Microbial biofilm |
Treatment:
Treatment ID: | TR002923 |
Treatment Summary: | A total of 15 microcosms were filled with 15 L of the Gave de Pau water; three for each of the five exposure conditions: 2 x 10-9 M (background concentration used as control), 1 x 10-7, 1 x 10-6, 5 x 10-6 and 1 x10-5 M Co. Cobalt (Cobalt Standard for ICP, 1000 mg·L-1, Supelco, Germany) was added in each microcosm and let equilibrating for 24 h (D-1). The microcosms were placed in one of the artificial streams of the facility in order to maintain the water temperature at 13.5 ± 1.0°C (Table S2). After one, three and seven days of exposure (D1, D3 and D7), slides were collected from each microcosm for metabolomics. |
Sample Preparation:
Sampleprep ID: | SP002920 |
Sampleprep Summary: | Briefly, freeze-dried biofilms (1 mg) were diluted in 10 µL of cold 75% methanol acidified with 0.1% formic acid and sonicated on ice during 30 s, at 80 % of the maximum intensity (SONICS Vibra Cell, Newton, CT, USA; 130 Watts, 20 kHz). The homogenates were then centrifuged at 4°C (12,000 g: 10 min). The supernatants were then collected and stored in the dark at -20°C. |
Processing Storage Conditions: | -20℃ |
Combined analysis:
Analysis ID | AN004564 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Thermo Acclaim Polar Advantage II (150 x 3mm,3um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Bruker maXis Impact qTOF |
Ion Mode | POSITIVE |
Units | Dalton |
Chromatography:
Chromatography ID: | CH003429 |
Instrument Name: | Waters Acquity |
Column Name: | Thermo Acclaim Polar Advantage II (150 x 3mm,3um) |
Column Temperature: | 40 |
Flow Gradient: | 5-90% |
Flow Rate: | 300 microliter per minute |
Solvent A: | Water |
Solvent B: | ACN |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004310 |
Analysis ID: | AN004564 |
Instrument Name: | Bruker maXis Impact qTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Metabolites were subsequently ionised and analysed using an electrospray ionization hybrid quadrupole time-of-flight high-resolution mass spectrometer (ESI-Qq-TOF Compact, Bruker) at 2 Hz speed on the 50–1500 m/z range on positive autoMS/MS mode between 2 and 8 Hz speed on the 50–1500 m/z range with information-dependent acquisition (IDA). |
Ion Mode: | POSITIVE |