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MB Sample ID: SA300148
| Local Sample ID: | Salvia-miltiorrhiza |
| Subject ID: | SU002902 |
| Subject Type: | Plant |
| Subject Species: | Salvia |
| Species Group: | Plants |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002902 |
| Subject Type: | Plant |
| Subject Species: | Salvia |
| Species Group: | Plants |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Salvia-miltiorrhiza | SA300148 | FL036425 | Salvia miltiorrhiza | Taxon |
Collection:
| Collection ID: | CO002895 |
| Collection Summary: | Samples were collected from live mature plants and separated into root and leaf samples, with 3 biological replicates. Then samples were frozen in liquid nitrogen. The 2 parts of each plant were crushed into homogeneous powders respectively. In total, 71 species from three major distribution centers for this genus representing 8 out of the 11 recognized subclades of Salvia were sampled. To obtain sufficient materials for both chemical analyses, samples were collected at the vegetative or flowering stages. |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR002911 |
| Treatment Summary: | No treatment, just species difference. In total, 71 species from three major distribution centers for this genus representing 8 out of the 11 recognized subclades of Salvia were sampled. |
Sample Preparation:
| Sampleprep ID: | SP002908 |
| Sampleprep Summary: | All samples were ground into a fine powder, and 10 mg prepared powder was extracted with 1 mL of 70% methanol (v/v) containing warfarin (5 μg/mL) as the internal reference for 1 h in an ultrasonic bath (53 kHz, 350 W) at 4 °C. After centrifuging at 12,000 g at 4 °C for 30 min, the supernatant was used for UHPLC-QTOF-MS analysis. |
Combined analysis:
| Analysis ID | AN004547 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Xevo-G2-XS |
| Ion Mode | UNSPECIFIED |
| Units | Normalized peak height |
Chromatography:
| Chromatography ID: | CH003416 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40 °C |
| Flow Gradient: | The elution gradients were 98–80% A over 0–7 min, 80–78% A over 7–11 min, 78–40% A over 11–20 min, 40–35% A over 20–25 min, 25–28 min at 35% A, 35–5% A over 28–30 min, 30–33 min at 5% A, and final re-equilibration at 98% A for 5 min. |
| Flow Rate: | 0.40 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004294 |
| Analysis ID: | AN004547 |
| Instrument Name: | Waters Xevo-G2-XS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS-DIAL v4.60 software. The data in the results table represent the average values for roots and leaves. Positive and negative ion-mode data were combined in the results table |
| Ion Mode: | UNSPECIFIED |
