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MB Sample ID: SA299605
| Local Sample ID: | 20210308-C1-4-72h |
| Subject ID: | SU002901 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Gender: | Female |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002901 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Gender: | Female |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 20210308-C1-4-72h | SA299605 | FL036384 | Control | Treatment |
Collection:
| Collection ID: | CO002894 |
| Collection Summary: | Whole blood was collected about 5 hours, 1 day, 2 days, 3 days, and 5 days before and after irradiation, and tissue samples were taken on the fifth day。 |
| Sample Type: | Blood (whole) |
Treatment:
| Treatment ID: | TR002910 |
| Treatment Summary: | After anesthesia with 10% chloral hydrate solution (0.25 ml/100 g) by intraperitoneal injection, the small animal radiation therapy simulator (X-RAD SmART) was used to irradiate the whole lung tissue using a 40×40 mm lead collimator. Whole chest X-ray irradiation of 10 Gy, 20 Gy and 35 Gy in each dose group was given by anterior-anterior-field cross-irradiation, and the dose rate was about 2.7 Gy/min. The control group was also anesthetized, but not irradiated |
Sample Preparation:
| Sampleprep ID: | SP002907 |
| Sampleprep Summary: | Plasma：Add 200 μL ice acetonitrile containing internal standard (internal standard and its concentration are shown in Table 1-3) to 50 μL plasma, swirl and mix well for 2 min, centrifuge at 13,000 rpm and 4 ℃ for 15 min, and divide the supernatant into two parts in parallel (100 μL each for determination and analysis of positive and negative ion modes of LC-MS). Freeze-dried and stored in a -80 ℃ refrigerator. Before metabolomics analysis, the samples were redissolved with 50 μL 25% acetonitrile/water (v/v) solution。 Tissue:Weigh about 20 mg lung tissue sample powder into 2 mL EP tube, add 1.5 mL 80% methanol/water (v/v) solution containing internal standard (internal standard and its concentration are shown in Table 2-1), swirl and mix well for 2 min, then add 3 zirconium beads into the sample and grind at 45 hz for 2 times, 5 min each time. The supernatant was divided into two parts in parallel (600 μL each for determination of positive and negative ion modes of LC-MS) at an interval of 15 s, and then centrifuged at 13,000 rpm for 15 min at 4 ℃. The supernatant was freeze-dried and stored in a refrigerator at -80 ℃. Before metabolomics analysis, samples were redissolved with 100 μL 80% methanol/water (v/v) solution. |
Combined analysis:
| Analysis ID | AN004545 | AN004546 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo TSQ Vantage HPLC-MS/MS | Thermo TSQ Vantage HPLC-MS/MS |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Thermo TSQ Vantage | Thermo TSQ Vantage |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH003414 |
| Chromatography Summary: | negtive ion mode |
| Instrument Name: | Thermo TSQ Vantage HPLC-MS/MS |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Gradient: | 0~1 min, 2% B; 1-20 min, 2~100% B; 20~24min, 100% B; 24~24.5min, 100~2% B; 24.5~34 min, 2% B |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003415 |
| Chromatography Summary: | positive ion mode |
| Instrument Name: | Thermo TSQ Vantage HPLC-MS/MS |
| Column Name: | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0~0.5min, 5% B; 0.5 ~24min, 5~100% B; 24-28 min, 40-100% B; 28~28.5min, 100~5% B; 28.5~48 min, 5% B |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | water(0.1% formic acid ) |
| Solvent B: | acetonitrile(0.1% formic acid ) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004292 |
| Analysis ID: | AN004545 |
| Instrument Name: | Thermo TSQ Vantage |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The temperature of ion source and ion transport tube is 350 ℃, the electrospray voltage in positive and negative ion mode is -2,500 V respectively, the sheath pressure is 35 Arb, and the auxiliary pressure is 10 Arb. Data acquisition and pre-processing and instrument interface control are performed in Xcalibur (Thermo, USA) software. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004293 |
| Analysis ID: | AN004546 |
| Instrument Name: | Thermo TSQ Vantage |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The temperature of ion source and ion transport tube is 350 ℃, the electrospray voltage in positive and negative ion mode is 3,000 V respectively, the sheath pressure is 35 Arb, and the auxiliary pressure is 10 Arb. Data acquisition and pre-processing and instrument interface control are performed in Xcalibur (Thermo, USA) software. |
| Ion Mode: | POSITIVE |
