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MB Sample ID: SA257903
Local Sample ID: | MCF0000858_SR22 |
Subject ID: | SU002664 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | WT/PMM2-CDG |
Age Or Age Range: | 5-45 |
Gender: | Male and female |
Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002664 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | WT/PMM2-CDG |
Age Or Age Range: | 5-45 |
Gender: | Male and female |
Species Group: | Mammals |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MCF0000858_SR22 | SA257903 | FL033108 | WT | Genotype |
MCF0000858_SR22 | SA257903 | FL033108 | 5.5mM 13C Glc siRNA | Treatment |
Collection:
Collection ID: | CO002657 |
Collection Summary: | Cells were washed with PBS, cells were incubated with extraction buffer for 2min before scraping and transferring to a fresh eppendorf. Samples were precipitated overnight at -80, then they were centrifuged at max rpm, 20min, 4 degrees C and supernatant transferred to an M/S vial. |
Sample Type: | Fibroblasts |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002676 |
Treatment Summary: | Cells were treated with vehicle or 5nM siRNA targeting AKR1B1 |
Sample Preparation:
Sampleprep ID: | SP002670 |
Sampleprep Summary: | Metabolites were extracted with 80% Methanol and IS (d27 myristic acid) |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |
Combined analysis:
Analysis ID | AN004224 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | NEGATIVE |
Units | AUC |
Chromatography:
Chromatography ID: | CH003133 |
Chromatography Summary: | C18 iP REVERSE PHASE |
Instrument Name: | Waters Acquity |
Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
Column Temperature: | 40 |
Flow Gradient: | The gradient started with 5% of solvent B and 95% solvent A and remained at 5% B until 2 min post injection. A linear gradient to 37% B was carried out until 7 min and increased to 41% until 14 min. Between 14 and 26 minutes the gradient increased to 95% of B and remained at 95% B for 4 minutes. At 30 min the gradient returned to 5% B. The chromatography was stopped at 40 min. |
Flow Rate: | 0.25 mL/min |
Solvent A: | 100% water; 10mM tributylamine; 15mM acetic acid |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003971 |
Analysis ID: | AN004224 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | El-Maven polly, ThermoFisher Xcalibur; Metabolites were annotated using the inhouse standard metabolite library- elution time and m/z values. |
Ion Mode: | NEGATIVE |