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MB Sample ID: SA234896
Local Sample ID: | FB0493 |
Subject ID: | SU002426 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002426 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
FB0493 | SA234896 | FL029160 | Male | Sex |
Collection:
Collection ID: | CO002419 |
Collection Summary: | Fecal samples from all participants were collected on the examination day, and then stored at -80℃ within four hours. |
Sample Type: | Feces |
Treatment:
Treatment ID: | TR002438 |
Treatment Summary: | N/A |
Sample Preparation:
Sampleprep ID: | SP002432 |
Sampleprep Summary: | The order of all test samples is randomly selected before the preparation. 10 mg lyophilized feces were homogenized with 25μL water and extracted with 185μL cold ACN-Methanol (8/2, v/v). After centrifugation, 30 μL supernatant was used to derivatization with 20 μL freshly prepared derivative reagents on a Biomek 4000 workstation (Biomek 4000, Beckman Coulter, Inc., Brea, California, USA), followed by mixing with internal standards. Subsequently, the derivatized samples and serial dilutions of derivatized stock standards were randomly analyzed and quantitated by an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) system (ACQUITY UPLC-Xevo TQ-S, Waters Corp., Milford, MA, USA). |
Combined analysis:
Analysis ID | AN003819 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters Xevo TQ-S |
Ion Mode | UNSPECIFIED |
Units | umol/L |
Chromatography:
Chromatography ID: | CH002826 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003561 |
Analysis ID: | AN003819 |
Instrument Name: | Waters Xevo TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Capillary (Kv):1.5 (ESI+), 2.0 (ESI-) Source Temp (°C):150 Desolvation Temp(°C):550 Desolvation Gas Flow (L/Hr):1000 MS acquisition software: QuanMET software (v2.0, Metabo-Profile, Shanghai, China) |
Ion Mode: | UNSPECIFIED |