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MB Sample ID: SA234484

Local Sample ID:FB0921
Subject ID:SU002426
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU002426
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
FB0921SA234484FL029159FemaleSex

Collection:

Collection ID:CO002419
Collection Summary:Fecal samples from all participants were collected on the examination day, and then stored at -80℃ within four hours.
Sample Type:Feces

Treatment:

Treatment ID:TR002438
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002432
Sampleprep Summary:The order of all test samples is randomly selected before the preparation. 10 mg lyophilized feces were homogenized with 25μL water and extracted with 185μL cold ACN-Methanol (8/2, v/v). After centrifugation, 30 μL supernatant was used to derivatization with 20 μL freshly prepared derivative reagents on a Biomek 4000 workstation (Biomek 4000, Beckman Coulter, Inc., Brea, California, USA), followed by mixing with internal standards. Subsequently, the derivatized samples and serial dilutions of derivatized stock standards were randomly analyzed and quantitated by an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) system (ACQUITY UPLC-Xevo TQ-S, Waters Corp., Milford, MA, USA).

Combined analysis:

Analysis ID AN003819
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Xevo-TQ-S
Ion Mode UNSPECIFIED
Units umol/L

Chromatography:

Chromatography ID:CH002826
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003561
Analysis ID:AN003819
Instrument Name:Waters Xevo-TQ-S
Instrument Type:QTOF
MS Type:ESI
MS Comments:Capillary (Kv):1.5 (ESI+), 2.0 (ESI-) Source Temp (°C):150 Desolvation Temp(°C):550 Desolvation Gas Flow (L/Hr):1000 MS acquisition software: QuanMET software (v2.0, Metabo-Profile, Shanghai, China)
Ion Mode:UNSPECIFIED
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