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MB Sample ID: SA229226
| Local Sample ID: | 191115_FW_1_project_Ermolaeva_1_10_PE035 |
| Subject ID: | SU002411 |
| Subject Type: | Invertebrate |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
| Age Or Age Range: | Young, middle aged and old |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002411 |
| Subject Type: | Invertebrate |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
| Age Or Age Range: | Young, middle aged and old |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 191115_FW_1_project_Ermolaeva_1_10_PE035 | SA229226 | FL028964 | ncl-1 RNAi | Treatment |
| 191115_FW_1_project_Ermolaeva_1_10_PE035 | SA229226 | FL028964 | 6 | Days of adulthood |
Collection:
| Collection ID: | CO002404 |
| Collection Summary: | Worms were washed and pelleted, M9 media was removed with ~100 µl remaining in the sample, and the sample was snap frozen. |
| Sample Type: | Worms |
Treatment:
| Treatment ID: | TR002423 |
| Treatment Summary: | Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) producing the corresponding dsRNA. Worms were cultured under standard conditions as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 animals were grown on RNAi until days 2, 6 or 12 of adulthood. |
Sample Preparation:
| Sampleprep ID: | SP002417 |
| Sampleprep Summary: | Phospholipids were extracted from C. elegans by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS. |
| Extract Storage: | -20℃ |
Combined analysis:
| Analysis ID | AN003793 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity H-Class |
| Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | relative units |
Chromatography:
| Chromatography ID: | CH002806 |
| Chromatography Summary: | Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC. |
| Instrument Name: | Waters Acquity H-Class |
| Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003535 |
| Analysis ID: | AN003793 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex). |
| Ion Mode: | NEGATIVE |
