Return to study ST002324 main page

MB Sample ID: SA229210

Local Sample ID:191115_FW_1_project_Ermolaeva_1_10_PG025
Subject ID:SU002411
Subject Type:Invertebrate
Subject Species:Caenorhabditis elegans
Taxonomy ID:6239
Age Or Age Range:Young, middle aged and old

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU002411
Subject Type:Invertebrate
Subject Species:Caenorhabditis elegans
Taxonomy ID:6239
Age Or Age Range:Young, middle aged and old

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
191115_FW_1_project_Ermolaeva_1_10_PG025SA229210FL028963ncl-1 RNAiTreatment
191115_FW_1_project_Ermolaeva_1_10_PG025SA229210FL0289632Days of adulthood

Collection:

Collection ID:CO002404
Collection Summary:Worms were washed and pelleted, M9 media was removed with ~100 µl remaining in the sample, and the sample was snap frozen.
Sample Type:Worms

Treatment:

Treatment ID:TR002423
Treatment Summary:Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) producing the corresponding dsRNA. Worms were cultured under standard conditions as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 animals were grown on RNAi until days 2, 6 or 12 of adulthood.

Sample Preparation:

Sampleprep ID:SP002417
Sampleprep Summary:Phospholipids were extracted from C. elegans by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS.
Extract Storage:-20℃

Combined analysis:

Analysis ID AN003793
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity H-Class
Column Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTRAP
MS instrument name ABI Sciex 5500 QTrap
Ion Mode NEGATIVE
Units relative units

Chromatography:

Chromatography ID:CH002806
Chromatography Summary:Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC.
Instrument Name:Waters Acquity H-Class
Column Name:Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003535
Analysis ID:AN003793
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex).
Ion Mode:NEGATIVE
  logo