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MB Sample ID: SA227158
| Local Sample ID: | 283N |
| Subject ID: | SU002400 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002400 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 283N | SA227158 | FL027584 | WT | Genotype |
Collection:
| Collection ID: | CO002393 |
| Collection Summary: | Liver was frozen on liqN2. Liver was crushed on liqN2, and metabolites extracted with 80% meOH. MeOH was dried, and samples derivatized to form methoxime-TBDMS adducts and immediately injected onto the GCMS. |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR002412 |
| Treatment Summary: | Mice were IP injected with a lactate/pyruvate (10:1) solution. |
Sample Preparation:
| Sampleprep ID: | SP002406 |
| Sampleprep Summary: | 80% methanol containing metabolites was dried overnight in a speedvac. Dried pellets were derivatized using methoxyamine and MTBSTFA. Samples were immediately injected. |
Combined analysis:
| Analysis ID | AN003781 |
|---|---|
| Chromatography ID | CH002795 |
| MS ID | MS003524 |
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5977A |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH002795 |
| Chromatography Summary: | 60C for 1 min then 10C/min to 320C for 1 min 9 min at 320C |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Injection Temperature: | 60 |
| Internal Standard: | Norvaline |
| Sample Injection: | Splitless |
| Chromatography Type: | GC |
MS:
| MS ID: | MS003524 |
| Analysis ID: | AN003781 |
| Instrument Name: | Agilent 5977A |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | Scan 75 - 1000 m/z Data processed using Agilent Enhanced Data Analysis Peaks were auto integrated and confirmed by hand. |
| Ion Mode: | POSITIVE |
