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MB Sample ID: SA227066
| Local Sample ID: | M4TP1 |
| Subject ID: | SU002399 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002399 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| M4TP1 | SA227066 | FL027575 | Control | Group |
| M4TP1 | SA227066 | FL027575 | Week 0 | Time point |
Collection:
| Collection ID: | CO002392 |
| Collection Summary: | Blood samples were collected from the tails of all animals at week 0 and a final cardiac puncture blood was taken at the termination of the study (week 8). |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -20℃ |
Treatment:
| Treatment ID: | TR002411 |
| Treatment Summary: | Each treatment group received one of four diets for the duration of the study, the dietary groups included; Control, which consisted of 100% AIN76 (Altromin, Germany). Sausage meat (15%)/AIN76 (85%), Frankfurter (15%)/AIN76 (85%), unprocessed pork (15%)/AIN76 (85%).All meat was freeze dried by European Freeze Dry (UK) and powderized. Altromin then incorporated this into pellet composed of 15% meat powder and 85% AIN76. |
Sample Preparation:
| Sampleprep ID: | SP002405 |
| Sampleprep Summary: | Samples were dispensed into a 96-well plate, dried at room temperature for 30 min and then 50 µL of phenylisothiocyanate (PITC) was added. After a further 20 min incubation at room temperature samples were dried under nitrogen for 1 h. Ammonium acetate (3 mM) was added, and the plate was shaken at room temperature for 30 min and centrifuged for 2 min at 500 g. Each well then had 150 µL transferred from it to a 96 deep well plate along with and equal volume of dH2O. |
Combined analysis:
| Analysis ID | AN003779 | AN003780 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class |
| Column | Biocrates Quant500 | Biocrates Quant500 |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Waters Xevo-TQ-S | Waters Xevo-TQ-S |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | uM | uM |
Chromatography:
| Chromatography ID: | CH002794 |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Biocrates Quant500 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003522 |
| Analysis ID: | AN003779 |
| Instrument Name: | Waters Xevo-TQ-S |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | ESI positive acquisition Data were acquired using Waters Masslynx and processed using the MetIDQ software. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003523 |
| Analysis ID: | AN003780 |
| Instrument Name: | Waters Xevo-TQ-S |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | ESI negative acquisition Data were acquired using Waters Masslynxand processed using the MetIDQ software. |
| Ion Mode: | NEGATIVE |
